DYNAMIC THRESHOLDING AND TISSUE DISSOCIATION OPTIMIZATION FOR CITE-SEQ IDENTIFIES DIFFERENTIAL SURFACE PROTEIN ABUNDANCE IN METASTATIC MELANOMA

Dynamic thresholding and tissue dissociation optimization for CITE-seq identifies differential surface protein abundance in metastatic melanoma

Abstract Multi-omics profiling by CITE-seq bridges the RNA-protein gap in single-cell analysis but has been largely applied to liquid biopsies.Applying CITE-seq to clinically relevant solid biopsies to characterize healthy tissue and the tumor microenvironment is an essential next step in single-cell translational studies.In this study, gating West

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Transient Electrophoresis of a Cylindrical Colloidal Particle

We develop the theory of transient electrophoresis of a weakly charged, infinitely long cylindrical colloidal particle under an application of a transverse or tangential step electric field.Transient electrophoretic mobility approaches steady electrophoretic mobility with time.We derive closed-form expressions for the transient electrophoretic mobi

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Determination of suitable field workdays for tillage operations at selected locations of Kaduna State, Nigeria

Suitable field workdays for agricultural machine operations (ploughing, harrowing and ridging) of some selected areas of Kaduna State in Nigeria were determined using soil moisture level and rainfall criteria.These involved the development of soil moisture budget and establishment of tractability criteria for each soil types assessed.Thirty (30) ye

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